Exposure to hyperbaric pressure alters ganglioside expression in rat liver following partial hepatectomy (CROSBI ID 513319)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Markotić, Anita ; Čikeš Čulić, Vedrana ; Tičinović Kurir, Tina ; Radić, Saša ; Zemunik, Tatijana ; Mesarić, Marko
engleski
Exposure to hyperbaric pressure alters ganglioside expression in rat liver following partial hepatectomy
Introduction: Remnant liver after partial hepatectomy (PH) demands an increased amount of oxygen to restore hepatic energy. Modulation of the ganglioside content of hepatocyte plasma membrane could provide a mean for upregulating cellular responsiveness to a variety of factors. Materials and Methods: Rat liver gangliosides were analysed by high perfomance thin layer chromatography (HPTLC) followed with immunostaining 54 h after PH. Non-operated and three different groups of operated rats were studied according to the hausing conditions before or after operation: recovering under normal ambient conditions after operation (1), treating with hyperbaric pressure before operation and recovering under normal ambient conditions (2 or preHB), and recovering under hyperbaric pressure after operation (3 or postHB). Results: Liver of preHB group showed the highest expression of ganglioside GD1a, a marker of a peculiar transition phase of liver regeneration. Same animals showed enhanced expression of the several gangliosides: GM2, GM1a, GD1b, GT1b, GalNAcGM1b, nLc4, nLc6 and nLc8. Conclusion: Since galactosyl- and N-acetylgalactosaminyltransferase catalyse final step of synthesys of GM2, GM1a, GD1b, GalNAcGM1b, nLc4, nLc6, and nLc8, our results point at possible role of galactosyl- and N-acetylgalactosaminyltransferase in regulation of the liver growth after PH.
gangliosides; hyperbaric oxygenation; immunostaining; partial hepatectomy
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Podaci o prilogu
119-x.
2004.
objavljeno
Podaci o matičnoj publikaciji
Periodicum Biologorum, vol 106, Supp 1
Zagreb:
Podaci o skupu
Fourth Croatian Congress of Pharmacology
poster
01.01.2004-01.01.2004
Split, Hrvatska