engleski

Kinetic modeling of amino acid oxidation catalyzed by a new D-amino acid oxidase from Arthrobacter protophormiae

D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was kinetically characterized using its natural substrate D- methionine and aromatic amino acid 3, 4-dihydroxyphenyl-D-alanine (D- DOPA). Kinetic measurement revealed high specificity of this enzyme to D-methionine (Km = 0.24 mM) and lower specificity on D-DOPA as a substrate (Km = 9.33 mM). The enzyme showed no activity to L-methionine and L-DOPA. Batch and repetitive batch experiments were performed with both substrates in the presence and absence of catalase for hydrogen peroxide decomposition.

D-amino acid oxidase ; enzyme kinetics ; modelling

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