hrvatski

HPLC određivanje katehina u sjemenkama grožđa Plavac mali cv.

Using HPLC technique on reversed-phase, the amounts of (+)-catechin and (-)-epicatechin in grape seeds of autochthonous dalmatian sort Plavac mali, were analysed during the growth season. To prevent interference between phenolic compounds, because many of them have similar UV absorption spectra, we fractionated acidic and neutral phenolic compounds on Sep Pak C18 cartridges. catechins were separated using HPLC with gradient solvent systemat 280 nm. The grape seeds were analysed during various sstages of ripening. The results show the highest concentrations of catechins inthe verasion. The level decrease quickly throughout maturation. The major flavan-3-ol monomer was (+)-catechin.

HPLC; katehin; Plavac mali

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