engleski

Detection of the viruses on selected lines of dwarf bean (Phaseolus vulgaris L. var. nanus Martens) using ELISA-test

Species from the genus Phaseolus, including Phaseolus vulgaris. can be naturally infected by viruses that can cause significant economic damage through the reduction of yield and quality. Some of the most important viruses of beans are bean common mosaic /BCMV), Bean common mosaic necrosis (BCMNV) and Bean yellow mosaic virus (BYMV). In breeding it is very important to determine presence of viruses in newly bred lines, specially viruses that are transmitted through bean seed. Material resistant or tolerant to viral infection is favored in breeding programs. Plants were visually examined for five years. The symptoms on newly bred lines of dwarf dry bean in the field were monitored. The frequency of viral infections was determined using ELISA-test. Results of these visual examinations and analysis enabled us to form a collection of newly bred lines of dwarf dry bean, which are less susceptible to viral infections. Determined health conditions of these lines of dwarf dry bean would be, in presence of the satisfactory production characteristics, decisive for further breeding in order to produce new cultivars.

BCMV; BCNMV; bean; BYMV; ELISA

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