engleski

Liposomes with superoxide dismutase protect A2182 cells against oxidative stress

Superoxide dismutase (SOD), an antioxidant enzyme, was encapsulated into liposomes in order to increase its releasing time and to facilitate its cellular penetration. Positively, neutrally, and negatively charged liposomes were prepared by the proliposome method. Differently charged liposomes were examined for the efficiency of delivery of Cu/Zn superoxide dismutase (CuZnSOD) to human lung epithelial cells, A2182, and their prospects of cell protection from oxidative agents. Untreated cells and cells pre-treated with liposome-encapsulated CuZnSOD were exposed to oxidative stress caused by xanthine/xanthine oxidase. Cellular antioxidant response was monitored for 4 or 24 h after the beginning of oxidative stress induced by the activity of superoxide dismutase (SOD) and total glutathione concentration. CuZnSOD-loaded liposomes increased the SOD activity of A2182 cells 24 h after treatment. The highest increase of cellular SOD, by 108%, was achieved using anionic liposomes. Exposure of untreated cells to oxidative stress increased the cellular glutathione level after 24 h. Cells pre-treated with liposome encapsulated CuZnSOD were protected from oxidative stress, as shown by the unchanged concentration of cellular glutathione.

Liposomes; superoxide dismutase; A2182 cells; oxidative stress

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