engleski

Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer.

The use of biotechnology, and therefore embryo transfer and manipulation with embryos in vitro, has became an integral part of animal reproduction. In order to further exploit this technique several oocyte aspiration methods for IVF from previously selected living cows are suggested. A method is described for transvaginal ovum pick-up (OPU) in two donor cows, stimulated with FHS/LH (Stimufol , Merial, France) for 3 consecutive days. Aspiration of the follicles was performed on Day 4 and procedure was repeated once a week for three consecutive weeks. The OPU system (Pie Medical, the Netherlands) consists of 3 components: an ultrasound scanner with a sector 5/7, 5 MHz tranducer, an aspiration pump and a needle guidance system. Donor cows were sedated and after additional relaxation by epidural anesthesia, follicles were punctured under echographic control. The number of oocytes (cumulus-oocyte complex = COC) collected using OPU varied from 1 to 6 (2.5 an average) and mean recovery rate was 44.1%. Fertilization rate after 24 hours of oocytes-sperm co-incubation was 53.3%. Culture in vitro in SOFaaBSA media rendered an average of 33.3% morulas and blastocysts of good quality, for ET or cryopreservation.

cow; follicle; aspiration; oocyte; production in vitro; embryo

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