engleski

Effect of indole glucosinolates from Cruciferae familyon mutagenicity and antimutagenicity in prokaryotic and eukaryotic test-systems

Glucosinolates are secondary plant metabolites widely distributed in plants of Cruciferae family (e.g. Brussels sprouts, broccoli, cauliflower, cabbage). It has been shown that these naturally plant compounds can be easily hydrolyzed to produce a range of physiologically active agents (isothiocyanate, nitrile and thiocyanate), some of them with a significant antimutagenic/anticarcinogenic effect. The aim of our investigation was to examine cytotoxic, mutagenic and antimutagenic effect of indole-3-carbinol, beta-indolylacetonitrile and extract of glucosinolates from cauliflower (CY), which endogenous myrosinase normally generates by hydrolysis of 3-indolylmethyl glucosinolate. To investigate the potential cytotoxic, mutagenic and antimutagenic effects of these compounds, we used prokaryotic test-system, bacteria Salmonella typhimurium (TA100 and TA98) with and without metabolic activation. The yeast Saccharomyces cerevisiae was chosed as the eukaryotic test-system to detect gene conversion and reverse gene mutation (strain D7) and induction of mitotic aneuuploidy (strain D61.M). The results showed that compounds used in our study had neither cytotoxic nor mutagenic effect. On the other side, they successfully inhibit the acting of standard mutagens. The investigations on possible inhibition of tumor cells growth in presence of indole glucosinolates, and consequently their anticarcinogenic effect are in progress.

mutagenicity; antimutagenicity; Salmonella typhimurium; indole glucosinolates

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