engleski

Immunohistochemical Study of the Midline Structures in Developing Mammalian Brain

Introduction: Developmental mechanisms and molecular entities that underlay crossing or intersection of fiber tracts and give direction to certain population of growing axons in the interhemispheric cerebral wall is still missing link in our understanding of ontogeny of mammalian brain. Materials & Methods: To address this issue we used classical histology and immunohistochemistry to examine (a) possible molecular interaction of midline structures of the telencephalon (cells and extracellular matrix) with growing axons of developing great cerebral commissure (corpus callosum) and (b) to examine molecular substrata for growing axons across midline, in human comparing with mouse. Results: Using several molecules which were previously classified as guidance molecules (Sema3A, ephrinA4, neogenin), or et list have influence on axonal outgrowth (CS-56) and several others which are typical neuronal (NeuN, TUJ1, MAP1b, SMI312), or glial markers, (GFAP, CD11b) we found different spatial expression pattern of these molecules in the structures adjacent to or in corpus callosum during precrossing, crossing and postcrossing temporal windows of developing corpus callosum. Conclusions: This work elucidate requirement of contemporaneous of number of different guidance mechanisms to achieve complexity of morphogenetic events in the interhemispheric cerebral wall during the formation of the corpus callosum.

corpus callosum; fetal telencephalon; semaphorin3A

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