engleski

Vegetative propagation of grapevine (Vitis vinifera L.) in vitro

Microclonal propagation of grapevine was established by shoot tip culture and accelerated development of axillary buds of cultivars: Alphonse Lavelee, Grenache, Muscat Adda, Palomino, Pinot white, Pinot gris, Ugni blanc, and rootstock hybrids 41B, 5BB, SO4. Excised shoots with 2-4 leaf primordia (0.5-2 mm in length) were cultured on an MS medium supplemented with 4.4 uM BA and 2.9 uM IAA. Multiplication rate of multiple shoot cultures was tested during the six subcultures (6 weeks each). Depending on the cultivar, 3-15 shoots per culture were obtained. Developed shoots 1.5-3 cm high rooted on the MS medium with 2.9-11.4 uM IAA. Plantlets in axenic cultures showed juvenile characteristics - shortage of tendrils and spiral phyllotaxy.

grapevine; in vitro; juvenile characteristics

Kategorija: Kongresno priopćenje