engleski

Frequency of human platelet antigen genotypes in children with arterial ischemic stroke

Arterial ischemic stroke (AIS) in children is a relatively rare disease (with incidence of 2.7 per 100, 000 children/year) not yet clearly understood and with multifactorial etiology. Besides well established risk factors for AIS, there are accumulating data indicating the importance of prothrombotic abnormalities due to defects in coagulation and fibrinolytic system, endothelial cells and platelets. Among these, the role of platelets has not been well studied. The aim of our study was to determine the frequency of human platelet antigen (HPA) genotypes in the group of children with AIS (confirmed by brain imaging), and to compare the data with those obtained for the control group. The AIS group consisted of 22 children (15 males, 7 females ; age at first onset 11 months to 16 years) whereas 26 children (20 males, 6 females ; aged from 2 to 18 years) were included in the control group. The genotypes of HPA-1, HPA-2, HPA-3 and HPA-5 were determined by sequence-specific primer polymerase chain reactions. The calculated allele frequencies were as follows: for AIS patients, HPA-1a/b 0.75/0.25, HPA-2a/b 0.86/0.14, HPA-3a/b 0.77/0.23, HPA-5a/b 0.89/0.11 ; and for control subjects HPA-1a/b 0.94/0.06, HPA-2a/b 0.92/0.08, HPA-3a/b 0.50/0.50, HPA-5a/b 0.89/0.11. No statistically significant differences in the frequencies of HPA-2 (p=0.5429) and HPA-5 (p=0.7729) between AIS patients and control subjects could be detected, while statistically significant differences were obtained for HPA-1 (p=0.0195) and HPA-3 (p=0.0118) allele frequencies. These results indicate the potential role of glycoprotein IIb/IIIa and HPA-1 and -3 polymorphisms in platelet activation, and possible involvement in the thrombotic event.

human platelet antigen; polymorphism; arterial ischemic stroke

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