engleski

Micropropagation of some Croatian endemic plant species

A rapid clonal propagation method for three Croatian endemic plant species: Fibigia triquetra (DC.) Boiss., Degenia velebitica (Deg.) Hay. and Centaurea ragusina L. has been developed. Shoots originated from aseptically germinated seeds collected on natural habitats were used for culture initiation. The highest multiplication rate in a 4-weeks-culture period was achieved on half strength MS medium supplemented with 2.9 mikroM gibberellic acid and 0.5 mikroM 6-benzylaminopurine (BA) in Fibigia triquetra (9.2 shoots per explant) and Degenia velebitica (5.2 shoots per explant) cultures, or with addition of 1.0 mikroM BA in Centaurea ragusina (4.6 shoots per explant) cultures. Excised shoots (2-4 cm) were rooted on the same basal medium supplemented with different concentrations of auxins indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and alpha -naphtaleneacetic acid (NAA). The highest rooting percentage was obtained with addition of IBA - 8.6 mikroM for Fibigia triquetra shoots, 4.9 mikroM for Degenia velebitica shoots and 2.5 mikroM for Centaurea ragusina shoots. Rooted plantlets of all three species were successfully transferred to potting soil and acclimatized to outdoor conditions.

Fibigia triquetra; Degenia velebitica; Centaurea ragusina; MS medium

nije evidentirano