engleski

Sequential Kinetic Resolution Catalysed by Halohydrin Dehalogenase

A sequential kinetic resolution catalyzed by halohydrin dehalogenase was employed for the synthesis of two valuable enantiopure building blocks. Resolution of methyl 4-chloro-3-hydroxybutanoate methylester using a Trp249Phe mutant of halohydrin dehalogenase yielded methyl 4-cyano-3-hydroxybutanoate methylester with 96.8% ee (40% yield) and methyl 4-chloro-3-hydroxybutanoate methylester with 95.2% ee (41% yield). This reaction is carried out in aqueous solution under mild conditions and provides access to a useful statin side-chain building block

sequential kinetic resolution; halohydrin dehalogenase; cyanide

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