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Influence of organic selenium food supplements and fasting on oxidative damage in different tissues of broiler chickens

The influence of organic selenium food supplements on lipid peroxidation and protein oxidation in different tissues of Ross broiler chickens of both sexes in response to 48 hours food deprivation were investigated. Seven days old chickens were randomly divided into two groups of 20 broilers each as following: standard diet fed control group and experimental group, pair-fed with the same diet supplemented with 0.3 ppm organic selenium (Sel-Plex® ; ; ; , Alltech). At age of 42 days, ten chickens of control and ten chickens of experimental group were sacrificed and liver, kidney and duodenum were removed. Other chickens were deprived of food for 48 hours with free access to tap water. After the period of fasting, ten chickens of control and ten chickens of experimental group were also sacrificed and the same tissues were removed. Liver, duodenum and kidney tissue homogenates were analysed spectrophotometricaly for lipid peroxide concentrations measured as thiobarbituric-acid reactive substances (TBARS) and protein carbonyl content (PCC). A complex pattern of changes was observed. The PCC in kidney and duodenum of experimental chickens at the end of fattening period was higher than in control broilers (P<0.05). As a result of the food deprivation, lower PCC was found in liver of both control and experimental group (P<0.05). Simultaneously, the values for experimental group was significantly lower than in the control group (P<0.05). Kidney PCC was increased after fasting only in control birds (P<0.05). The TBARS concentration in kidney at the end of fattening was significantly higher in chickens fed diet supplemented with organic selenium (P<0.05). Kidney and duodenal tissue TBARS concentrations were decreased after food deprivation of both control and experimental group (P<0.05). At the same time TBARS concentration in liver of experimental group was increased (P<0.05). Results of this study demonstrated that dietary organic selenium supplements increased lipid peroxidation in kidney and protein oxidation in kidney and duodenum at the end of fattening. Food deprivation resulted in decreased tissue oxidative damage.

chicken; organic selenium; food deprivation; lipid peroxidation; protein oxidation

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