engleski

The effect of chratoxin A on the activity of enzymes in the basal-lateral membranes of the proximal tubules of rat kidney

Ochratoxin A (OA) is a mycotoxin that is produced by storage moulds and could be found on a variety comodities. Maximal concentrations that were reported in food of plant and of animal origins were 27.5 mg/kg and 67 ug/kg, respectively. Numerous studies have shown thet OA is a potent immunosupresive, carcinogenic, teratogenic and genotoxic agent. However, the most important toxic effects are found in the kidneys where an increase concentrations of OA are producing tubulo-interstitial nephropathy. LD50 for rats is 22-44 mg/KG b.w. The main goal of this study was to determine the effects of low doses of OA on the rat kidney. Daily dose of Oa (120ug/kg b. w.) was administrated to rats of Fisher strin during 10 and 35 days. Activities of Na, k-ATPase (the specific marker enzyme of basal lateral membranes), gama-glutamiltransferase and alkaline phosphatase were measured in the basal-lateral membranes of kidney proximal tubule. The concentration of OA in kidney was found to be 32 ng/g tissue and 48ng/g tissue after 10 and 35 days of experiment, respectively. Treatment lasting for 10 days produced a significant decrease in the activity of gamma-glutamiltransferase (reduction to 60% in comparison to the control value) and in the activity of alkaline phosphatase (reduction to 63%). However, the activity of Na, K-ATPase was increased to 124% after the 35 days of treatment.

Ochratoxin A; proximal tubules

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