engleski

Detection of micronuclei in haemocytes of freshwater snail Planorbarius corneus L. (Gastropoda, Pulmonata) exposed to pentachlorophenol

The aim of this study was to investigate the effect of worldwide pollutant pentachlorophenol (PCP) on the induction of micronuclei (MN) in haemocytes of freshwater snail Planorbarius corneus L. . Freshwater snails were exposed to different concentrations of PCP (10 mg/l; 100 mg/l, 450 mg/l; 600 mg/l) under laboratory conditions. The test solutions and water were changed daily during the 14 days of experiment. The samples of haemolymph were collected after 4, 7 and 14 days of exposure to PCP. The haemocytes were fixed in 1% glutaraldehyde and stained in 10 mg/ml Hoechst. The level of MN frequencies was expressed as the number of MN in 1000 cells (o/oo). The spontaneous level of MN frequencies ranged from 0.995 o/oo to 2.006 o/oo. Positive response (15.730 o/oo) was observed after 4 days of exposure to 600 mg/l PCP with the level of MN frequency 5 to 7 times higher than observed in other samples (from 2.006 o/oo to 3.413 o/oo). After 7 days of exposure the effect was dose-related, and the MN frequencies ranged from 7.928 o/oo (for 100 mg/l) and 8.964 o/oo (for 450 mg/l) to 13.972 o/oo (for 600 mg/l). After 14 days of exposure the frequency of MN ranged from 0.995 o/oo (for control) to 15.974 o/oo (for 450 mg/l) while in sample exposed to 600 mg/l the number of cells was too low to make the proper analysis. This test was also time-dependent for lower concentrations of PCP (100 and 450 mg/l). The frequency of MN significantly increased after 7 and 14 days of exposure with both concentrations.

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