hrvatski

RAZVOJNO SPECIFIČNI SASTAV PROTEINA I GLIKOPROTEINA U KULTURI TKIVA HRENA Armoracia lapathifolia Gilib.

In vitro grown horse-radish (Armoracia lapathifolia Gilib.) plantlets, tumour and teratoma tissues were compared with regard to protein and glycoprotein patterns. Crown-gall tumours were induced on the leaf fragments with wild octopine strain B6S3 of Agrobacterium tumefaciens. From the same primary crown-gall tumour two tissue lines were established: the shoot producing teratoma line (TM) and unorganised tumour line which never expressed any morphogenic capacity (TN). Leaf, teratoma and tumour cellular proteins were separated by SDS-PAGE as well as by 2D electrophoresis and stained with Coomassie brilliant blue or with silver-nitrate. Concavalin A-peroxidase staining detected cellular glycoproteins with D-mannose and D-glucose in their glycan component on 1D protein blots. For further characterisation of glycan component, digoxigenin-labeled lectins GNA, DSA, PNA, MAA and SNA were applied. Developmentally specific proteins of different horse-radish tissue lines were detected by SDS-PAGE, which revealed the 110, 70, 38 and 23 kDa polypeptides only in leaf extracts. Teratoma and tumour tissues had specific proteins of 115, 33 and 20 kDa while the 65 kDa polypeptide was characteristic for teratoma tissue. The glycoprotein of about 66 kDa detected by Con A was present in the leaf and tumour while the 45 kDa one was quantitatively dominant in the teratoma and tumour tissues. All of the Con A-reacting glycoproteins were also detected with GNA, which recognizes terminal D-mannose groups, especially those possessing Man-α (1-3)-Man units present in high-mannose type glycans. The 2D electrophoresis revealed some tissue specific protein groups that have to be closer analyzed.

biljni razvitak; glikoproteini; hren; kultura biljnog tkiva; proteini

nije evidentirano

engleski

DEVELOPMENTALLY SPECIFIC PROTEIN AND GLYCOPROTEIN PATTERNS IN HORSE-RADISH Armoracia lapathifolia Gilib. TISSUE CULTURE

In vitro grown horse-radish (Armoracia lapathifolia Gilib.) plantlets, tumour and teratoma tissues were compared with regard to protein and glycoprotein patterns. Crown-gall tumours were induced on the leaf fragments with wild octopine strain B6S3 of Agrobacterium tumefaciens. From the same primary crown-gall tumour two tissue lines were established: the shoot producing teratoma line (TM) and unorganised tumour line which never expressed any morphogenic capacity (TN). Leaf, teratoma and tumour cellular proteins were separated by SDS-PAGE as well as by 2D electrophoresis and stained with Coomassie brilliant blue or with silver-nitrate. Concavalin A-peroxidase staining detected cellular glycoproteins with D-mannose and D-glucose in their glycan component on 1D protein blots. For further characterisation of glycan component, digoxigenin-labeled lectins GNA, DSA, PNA, MAA and SNA were applied. Developmentally specific proteins of different horse-radish tissue lines were detected by SDS-PAGE, which revealed the 110, 70, 38 and 23 kDa polypeptides only in leaf extracts. Teratoma and tumour tissues had specific proteins of 115, 33 and 20 kDa while the 65 kDa polypeptide was characteristic for teratoma tissue. The glycoprotein of about 66 kDa detected by Con A was present in the leaf and tumour while the 45 kDa one was quantitatively dominant in the teratoma and tumour tissues. All of the Con A-reacting glycoproteins were also detected with GNA, which recognizes terminal D-mannose groups, especially those possessing Man-α (1-3)-Man units present in high-mannose type glycans. The 2D electrophoresis revealed some tissue specific protein groups that have to be closer analyzed.

plant development; glycoproteins; horse-radish; proteins; plant tissue culture

nije evidentirano