engleski

Enzyme-linked immunosorbent assay in serological diagnosis of swine pleuropneumonia in Croatia

The present study was the first in Croatia which employed ELISA to establish the presence of antibodies against Actinobacillus pleuropneumoniae serovars 2 and 4-7 in the sera of breeding swine. A total of 782 sera were tested. The sera had been taken from 345 gilts (aged 6 months), 334 sows (over 12 months of age) and 103 boars (aged 6-12 months). Sera samples, taken from the animals kept at nine large pig breeding farms were examined for the presence of antibodies against A. pleuropneumoniae serovar 2. The results showed that infection with this serovar existed at seven of nine farms. The percentage of serologically positive gilts for serovar 2 at the seven infected farms ranged between 61% and 91%, of serologically positive sows from 26% to 61% and boars from 9% to 50%. Sera from farm 7, 8 and 9 were also examined for the presence of antibodies against serovar 4-7 ; findings were positive. The percentage of serologically positive gilts for A. pleuropneumoniae serovar 4-7 at these farms ranged from 78% to 91%, of sows from 74% to 78% and boars from 20% to 50%. ELISA was shown to be reliable method in the serological diagnosis of swine pleuropneumonia in breeding swine of various age, on a farm level.

swine pleuropneumonia; serological diagnosis; ELISA; Croatia

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