hrvatski

Effect of environmental conditions variation on biological stress indicators in mussel M. galloprovincialis

In the present work we investigated under experimental conditions progressive acclimation of mussel M. galloprovincialis to different lower salinity during high seawater (SW) temperature and its effect on several biochemical markers and biotests. Mussel M. galloprovinicialis were purchased from Limski kanal (Northern Adriatic, Croatia) in summer (SW temperature 27oC, salinity 37.5 psu). DNA integrity, induction of stress proteins and metallothioneins were measured and mussels were tested (survival in air) at the beginning and at the end of acclimation. Mussels were finally acclimated during 14 days to 37.0, 28.5, 18.5 and 11 psu, what correspond approximately 100, 75, 50 and 30% of SW. Survival in air (SOS) as a physiological index of mussel health and vitality had significantly lower LT50 values, and seems to be negatively affected by acclimation in comparison to control (100% SW and Limski kanal), except mussels acclimated to 50% SW. Lower DNA integrity determined by Fast Micromethod proved impact of salinity acclimatization and corresponding physiological stress on DNA level as well. Significant differences of stress-70 proteins contents in mussels’ gills of control groups and mussels exposed finally to 50 and 30% SW were established (HSP70 increase and HSP72 decrease). Contents of metallothionens in gills first reduced (75 and 50% SW) and then significantly increased (30% SW). Results indicated that SW salinity variation in marine environment can affect all investigated parameters. This investigation expands our understanding of multifactorial effects of the physical environment on the specificity of investigated biomarkers and biotests, providing insight into the stress response processes of mussel how it might be disrupted by environmental stresses and pollution.

M. galloprovincialis; biomarkeri; učinak kolebanja čimbenika okoliša

nije evidentirano

engleski

Effect of environmental conditions variation on biological stress indicators in mussel M. galloprovincialis

In the present work we investigated under experimental conditions progressive acclimation of mussel M. galloprovincialis to different lower salinity during high seawater (SW) temperature and its effect on several biochemical markers and biotests. Mussel M. galloprovinicialis were purchased from Limski kanal (Northern Adriatic, Croatia) in summer (SW temperature 27oC, salinity 37.5 psu). DNA integrity, induction of stress proteins and metallothioneins were measured and mussels were tested (survival in air) at the beginning and at the end of acclimation. Mussels were finally acclimated during 14 days to 37.0, 28.5, 18.5 and 11 psu, what correspond approximately 100, 75, 50 and 30% of SW. Survival in air (SOS) as a physiological index of mussel health and vitality had significantly lower LT50 values, and seems to be negatively affected by acclimation in comparison to control (100% SW and Limski kanal), except mussels acclimated to 50% SW. Lower DNA integrity determined by Fast Micromethod proved impact of salinity acclimatization and corresponding physiological stress on DNA level as well. Significant differences of stress-70 proteins contents in mussels’ gills of control groups and mussels exposed finally to 50 and 30% SW were established (HSP70 increase and HSP72 decrease). Contents of metallothionens in gills first reduced (75 and 50% SW) and then significantly increased (30% SW). Results indicated that SW salinity variation in marine environment can affect all investigated parameters. This investigation expands our understanding of multifactorial effects of the physical environment on the specificity of investigated biomarkers and biotests, providing insight into the stress response processes of mussel how it might be disrupted by environmental stresses and pollution.

M. galloprovincialis; biomarkers; effect of environmental conditions variation

nije evidentirano