DNA repair in Deinococcus radiodurans: from hundreds of fragments to the functional chromosome (CROSBI ID 519576)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Zahradka, Ksenija ; Radman, Miroslav
engleski
DNA repair in Deinococcus radiodurans: from hundreds of fragments to the functional chromosome
Bacterium Deinococcus radiodurans is one of the most radiation resistant organisms known. It can sustain extreme levels of ionizing radiation breaking its genome into hundreds of shorts fragments. Remarkably, in just couple of hours these fragments are reassembled into functional chromosomes due to an efficient and precise DNA repair process. We found that this repair involves a special kind of « ; ; extended synthesis-dependent strand annealing» ; ; (ESDSA) followed by homologous recombination. In ESDSA, chromosomal fragments produced by radiation are used both as primers and templates for a massive synthesis of long single strand extensions which then anneal thereby accurately connecting contigous DNA fragments into long linear intermediates. These intermediates are finally matured by RecA-dependent homologous recombination into functional circular chromosomes that are patchworks of old (synthesized before radiation) and new (synthesized after radiation) double-stranded DNA blocks.
Deinococcus radiodurans; DNA repair; homologous recombination; DNA synthesis
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nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
59-60-x.
2006.
objavljeno
Podaci o matičnoj publikaciji
Zbornik sažetaka 9. hrvatskog biološkog kongresa s međunarodnim sudjelovanjem
Besendorfer, Višnja ; Klobučar, Goran
Zagreb: Hrvatsko biološko društvo
Podaci o skupu
9. Hrvatski biološki kongres s međunarodnim sudjelovanjem
pozvano predavanje
23.09.2006-29.09.2006
Rovinj, Hrvatska