engleski

Molecular identification of Campylobacter spp. strains isolated from poultry in Croatia

Campylobacteriosis is the second reported food- borne infection in Croatia (www.publichealth-zagreb.hr), and poultry meat appears to be a major source of infection. Because of enormous impact of Campylobacter spp. on human health, the aim of this study was to evaluate the prevalence of C. coli and C. jejuni by using multiplex PCR (mPCR) in altogether 31 broiler and 28 laying hen cloacal swabs. After isolation on modified charcoal cefoperazone desoxycholate agar (mCCDA) plates (Oxoid, Unipath, UK), the DNA was extracted as described by Jackson et al. (1993). The identification of strains was obtained by the mPCR as described by Manfreda et al. (2003). Altogether 25 (80.64%) broiler samples and 17 (60.71%) laying hen samples were positive, whereas 16 (64%) of broilers and 10 (58.82%) of hens positives were detected as C. coli and 9 (36%) of broilers and 7 (41.17%) of hens as C. jejuni. The mPCR, based on the use of different sets of primers in one reaction is simple and time-saving method. Further investigation will be conducted on larger number of samples to reveal the real prevalence of both Campylobacter strains.

Campylobacter spp.; molecular identification; poultry; Croatia

nije evidentirano