engleski

Age-related changes in serum dipeptidyl peptidase IV (DPP IV/CD26) activity in patients with inflammatory bowel diseases

The dipeptidyl peptidase IV (DPP IV/CD26) is a membrane-bound multifunctional glycoprotein, acting as a proteolytic molecule, receptor, binding and costimulatory molecule. The proteolytic cleavage of the membrane bound DPP IV results in a soluble form that migrates in the plasma and other biological fluids. Inflammatory bowel diseases (IBDs) represent a group of idiopathic diseases which affect the intestine and include two clinical subtypes: Crohn's disease (CD) and ulcerative colitis (UC). The etiology and pathogenesis of IBD are still not understood well. It has previously been shown that the molecule CD26 (DPP IV/CD26) plays a significant role in the pathophysiology of IBD. The aim of this study was to determine the influence of age on the serum dipeptidyl peptidase IV (DPP IV/CD26) activity in patients affected with IBD and healthy controls. The research was performed on 93 patients, divided in 2 groups: 31 young patients (mean age 13, 8 +/- 1, 7 years, 24 with CD and 7 with UC) and 62 adult patients (age 42, 7 +/- 14, 4 years, 38 with CD and 24 with UC). The control group included 111 healthy blood donors: 46 children (age 13, 8 +/- 2, 8 years) and 65 adults (age 41, 6 +/- 12, 1 years). The serum DPP IV activities in both young and adult patients with IBD were found to be statistically sigificantly decreased when compared to their healthy controls. The values correlated inversely with the disease severity for both CD and UC. When comparing serum DPP IV activities between young and adult patients with IBD, but even between young and adult healthy controls, it is clear that the serum DPP IV activity decreases statistically significantly with age. The results of this study also indicate that the serum DPP IV activity could be useful as an available, non-invasive marker in the diagnosis of the disease activity. This research shows that age-related standard values should be established in clinical laboratory practice because of the age-dependent decrease of DPP IV/CD26 activity in serum.

Dipeptidyl peptidase IV (DPP IV/CD26); Crohn's disease; Serum

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