Electrochemical methods for quantification and characterization of metallothioneins induced in Mytilus galloprovincialis (CROSBI ID 77831)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Raspor, Biserka ; Pavičić, Jasenka
engleski
Electrochemical methods for quantification and characterization of metallothioneins induced in Mytilus galloprovincialis
The quantification of the purified metallothionein (MT) component, isolated from the digestive gland of cadmium-exposed Mytilus galloprovincialis , is described based on the analysis of Cd(II) and SH-groups content, applying electrochemical methods. Advantages and disadvantages of the Brdi~ka procedure for the determination of the MT content is discussed. The saturation of binding positions of purified MT with Cd2+ ions can be directly followed voltammetrically. Irrespective of the MT concentration, the saturation with Cd2+ of in vivo induced mussel MT is achieved at a molar ratio of 5. Cd2+ ions are rapidly displaced from the Cd-Th complex after the addition of Pb2+ ions, which indicates the kinetically labile type of the complex.
metallothioneins ; quantification of ; characterization of
Conference: 6th International Hans Wolfgang Nurnberg Memorial Symposium on Metal Compounds in Environment and Life Location: FORSCHUNGSZENTRUM JULICH, JULICH, GERMANY Date: MAY 09-12, 1995
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Podaci o izdanju
354 (5-6)
1996.
529-534
objavljeno
0937-0633
10.1007/s0021663540529