The selective effect of propolis and its polyphenolic compounds on primary culture of human urinary bladder cancer and normal epithelial cells (CROSBI ID 520572)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Oršolić, Nada ; Štajcer, Damir ; Šver, Lidija ; Kosalec, Ivan ; Bakmaz, Marina ; Terzić, Svjetlana ; Bašić, Ivan
hrvatski
The selective effect of propolis and its polyphenolic compounds on primary culture of human urinary bladder cancer and normal epithelial cells
Large amounts of epidemiological data have supported the inverse relation between the consumption of fruits and vegetables and the incidence of cancer. The flavonoids present in propolis are considered to be rich source of chemopreventive agents, since they have various therapeutic biological activities. This study was carried out to find whether propolis and its polyphenolic/flavonoids compounds may induce cytotoxicity in primary culture of human urinary bladder cancer cells as compared to normal urinary bladder epithelial cells. Pieces of urinary bladder cancer grown in the cavity of urinary bladder as well as normal epithelial tissue from urinary bladder wall were taken from patients in different stages (grade G1, G2, G3) of bladder carcinoma. Single cells suspension from pieces of either cancer or normal tissue was prepared according standard laboratory procedure. Incubation for cytotoxicity testing were carried out in RPMI-1640 medium with 20% FCS at 37 ˚ C with 5% CO2 with or without different concentration (50, 150, 300 µ ; ; g/ml) of test components. The cytotoxicity of two preparations of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, and quercetin) was determined using trypan blue exlusion assay. These findings suggest that EEP is the most effective in inhibition of urinary bladder cancer cell proliferation as compared to flavonoids derived from propolis. All test components have no cytotoxic effect on normal epithelial cells. The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to improve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation have expressed no side effect after treatment. However, the exact cancer chemoprevention mechanisms of propolis effectiveness have to be elucidated.
propolis; tumor; mokraćni mjehur
nije evidentirano
engleski
The selective effect of propolis and its polyphenolic compounds on primary culture of human urinary bladder cancer and normal epithelial cells
Large amounts of epidemiological data have supported the inverse relation between the consumption of fruits and vegetables and the incidence of cancer. The flavonoids present in propolis are considered to be rich source of chemopreventive agents, since they have various therapeutic biological activities. This study was carried out to find whether propolis and its polyphenolic/flavonoids compounds may induce cytotoxicity in primary culture of human urinary bladder cancer cells as compared to normal urinary bladder epithelial cells. Pieces of urinary bladder cancer grown in the cavity of urinary bladder as well as normal epithelial tissue from urinary bladder wall were taken from patients in different stages (grade G1, G2, G3) of bladder carcinoma. Single cells suspension from pieces of either cancer or normal tissue was prepared according standard laboratory procedure. Incubation for cytotoxicity testing were carried out in RPMI-1640 medium with 20% FCS at 37 ˚ C with 5% CO2 with or without different concentration (50, 150, 300 µ ; ; g/ml) of test components. The cytotoxicity of two preparations of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, and quercetin) was determined using trypan blue exlusion assay. These findings suggest that EEP is the most effective in inhibition of urinary bladder cancer cell proliferation as compared to flavonoids derived from propolis. All test components have no cytotoxic effect on normal epithelial cells. The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to improve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation have expressed no side effect after treatment. However, the exact cancer chemoprevention mechanisms of propolis effectiveness have to be elucidated.
propolis; tumor; urinary bladder
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
244-246.
2006.
objavljeno
Podaci o matičnoj publikaciji
9. hrvatski biološki kongres s međunarodnim sudjelovanjem : Zbornik sažetaka = 9th Croatian Biological Congress with International Participation : Proceeding of abstracts
Besendorfer, Višnja ; Klobučar, Goran I. V.
Zagreb: Hrvatsko biološko društvo
953-6241-06-4
Podaci o skupu
Hrvatski biološki kongres s međunarodnim sudjelovanjem (9 ; 2006=
pozvano predavanje
23.09.2006-29.09.2006
Rovinj, Hrvatska