engleski

Cytotoxicity of propolis and its polyphenolic compounds on primary culture of human urinary bladder transitional cell carcinoma.

The flavonoids present in propolis are considered to be rich source of chemopreventive agents, since they have various therapeutic biological activities. This study was carried out to find whether propolis and its polyphenolic/flavonoids compounds may induce cytotoxicity in primary culture of human urinary bladder transitional cell carcinoma (TCC) cells as compared to normal urinary bladder epithelial cells. Pieces of TCC or normal epithelial tissue were collected by transurethral surgery from patients in different stages (grade G1, G2, G3) of TCC. Single cells suspension from pieces of either TCC or normal bladder epithelial tissue was prepared according standard laboratory procedure. Incubation for cytotoxicity testing were carried out in RPMI-1640 medium with 20% FCS at 37 ˚ C with 5% CO2 with or without diffrent concentration (50, 150, 300 µ ; ; g/ml) of test components. The cytotoxicity of two preparation of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its related polyphenolic compounds (caffeic acid, naringin, chrysin, quercetin) was determined using trypan blue exlusion assay. Findings suggest that EEP is the most effective in inhibition of urinary bladder TCC cell proliferation as compared to WSDP or single flavonoids present in propolis. All test components showed no cytotoxic effect on normal epithelial cells (Figure 1). The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to impruve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation or its polyphenolic components have expressed no side effect after treatment. However, the exact cancer chemoprevention mechanisms of propolis effectivness have to be elucidated.

propolis; tumor; urinary bladder

Rad je kao predavanje prezentiran i na skupu "7th EAU Central European Meeting", održanom od 26.-27. 10.2007., Zagreb, Hrvatska, a objavljen kao sažetak u časopisu, ISSN 1872-7174 ; str. 18-18.