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Genetic diversity of indigenous Bradyrhizobium japonicum soil population in Croatia

The aim of this investigation was to identify and to characterize indigenous Bradyrhizobium japonicum strains isolated from different soil types in eastern Croatia which are regularly used for agricultural production. The actual composition and genetic diversity of natural field population were studied using RAPD fingerprinting. Total genomic DNAs from 19 field isolates and two reference strains from commercial inoculant were amplified using six different 10-mer primers. Characteristic and informative band patterns were obtained for all strains analyzed. RAPD profiles revealed that indigenous B. japonicum strains were predominant in field population. Only a few isolates corresponded to inoculant strain. One soil sample contain both indigenous and inoculant strains while the others contain exclusively indigenous bradyrhizobia. Among indigenous B. japonicum strains several distinct groups can be determined.

genetic diversity; B. japonicum; soil population; RAPD fingerprinting

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