engleski

Interspecific and interindividual variability of NORs and ITS restriction pattern in Quercus species

Fluorochrome banding was employed to characterize oak chromosomes. The karyotypes of three oak species, Quercus robur, Q. petraea and Q. cerris, showed interspecific uniformity of DAPI banding pattern. Chromomycin A3 (CMA) staining revealed CMA-positive bands on several chromosomes and two CMA-positive signals on the satellite chromosome pair. FISH confirmed that they corresponded to nucleolar organizing regions and satellites. Interindividual and even intercellular size variability of CMA-positive signals was observed. Satellites were often detached from the corresponding chromosome pair, and fusion occured occasionally between satellites, either detached or located on the chromosomes. The internal transcribed spacer (ITS) region was successfully amplified in Q. robur, Q. petraea, Q. cerris and Q. pubescens. The PCR-amplified ITS region was approximately 720 bp. Digestion of ITS with Alu I allowed us to distinguish Q. petraea from the other species investigated, while Hae III restriction patterns distinguished Q. robur and Q. cerris from other two species.

Quercus; fluorochrome banding; FISH; internal transcribed spacer (ITS); nucleolar organizer region

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