engleski

Biosynthesis and biodegradation of aflatoxin B1 and G1 on maize contaminated with common field and storage fungi

The relationship between mold biomass and the biosynthesis of aflatoxin B1 and 1 (AFB1 and AFG1) on solid substrates (whole and crushed maize grain) at temperatures from 15-35 oC and a water content in the substrate of 20-38% has been investigated. The experiments have been carried out with the mold Aspergillus flavus ATCC 26949 in pure culture and in mixed culture respectively, the latter with the molds Trichothecium roseum ZMPBF 1226 and usarium graminearum ZMPBF 1215. The biomass growth during cultivation was measured by the chitin content, and the concentration of aflatoxins was determined by HPLC method. It has been established that the biosynthesis of the examined aflatoxins and their ratio primarily depend on the temperature of cultivation, rather than on the growth of the mycelium. The biomass of the mixed culture of A. flavus, T. roseum and F. graminearum after 35 days of cultivation reduces the amount of AFB1 by 22-45%, and the amount of AFG1 by 36-48%. The decrease of concentration of both toxins is more pronounced in the substrate with a higher initial water content and a higher temperature of cultivation.

Molds; mycotoxins; mixed mold cultures; chitin; biomass

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