engleski

Antifungal and antimycotoxigenic properties of newly synthesized derivatives of coumarine

The potential for inhibition of aflatoxin accumulation by aflatoxigenic fungus Aspergillus parasiticus NRRL 2999 using the 4-chlorocoumarin-3-carbonitrile in yeast-extract-sucrose (YES) medium at pH 5.5.YES medium was treated with various amounts of this compound after inoculation with A. parasiticus. Experiments were carried out in a stationary culture at temperatures of 20 and 28 oC during 28 days.Mycelial dry weights were determined gravimetrically, and concentration of aflatoxin 1 and G1 (AFB1 and AFG1) were measured by HPLC method.Concentrations of investigated compound of 0.05 mmol x L-1 and 0.1 mmmol x L-1, respectively, stimulated mold growth and AFB1 and AFG1 accumulation, but concentrations higher than 0.2 mmol x L-1 produced an inhibitory effect.In the presence of 0.3 mmol x L-1 of investigated coumarine, mold growth was decreased by 55% and toxin concentrations by 85% or completely.

Molds; mycotoxins; antifungal agents; coumarines

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