engleski

Genetic diversity of Bradyrhizobium japonicum field population revaled by RAPD fingerprinting

RAPD fingerprinting was used for strain identification and the assesment of genetic diversity within a field population of Bradyrhizobium japonicum. Total genomic DNAs from 13 field isolates and two inoculant strains were amplified using six different 10-mer primers. Different and informative band patterns were obtained for all strains analysed. Cluster analysis unexpectedly revealed that none of the field isolates was identical to inoculant strains which were regurarly used for soybean inoculation. Among field isolates two highly divergent groups were determined. The results indicate that RAPD is very discriminative and efficient method for differentiating and studying genetic diversity of B. japonicum strains.

nitrogen fixation; Bradyrhizobium japonicum; indigenous strains; RAPD fingerprinting

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