engleski

Chromosome analysis in children with neuroblastoma

Neuroblastoma (NB) is the most common malignant solid tumor in children, but little is known about the etiology, prevention and effective monitoring the course of the disease. A special attention is paid to the investigation of biologic significance of constitutionala and acquired genomic changes in patients with neuroblastoma. In this study we present the results of cytogenetic analysis in our group of 20 children with NB. The aim of this study is: a) to determine the constitutional variability of C-heterochromatin of chromosomes 1, 9 and 16 in children with NB and to compare it to healthy control. b) to determine the frequency and types of acquired genomic abnormalities in our group of children with neuroblastoma.The analysis og C-heterochromatin variability was carried out on slides obtained by routine method of peripheral blood culture while the analysis of malignant cells was performed on tumor pieces, lymph node or bone marrow cells. A normal constitutional karyotype was detected in all children with NB. Statistically significant differences between the patients and the normal control were observed by studying C-segment length and localization variants of chromosomes 1, 9 and 16. Chromosome analysis of malignant cells revealed complex karyotype abnormalities including numerical and structural changes. Aberrations of chromosomes No.1 were the most frequent clonal rearrangements, as expected. this study confirms the karyotype heterogeneity of NB and revealed some rare cytogenetic aberrations. Additional investigations ere necessary to provide better understanding of the role of certain chromosome region in the genesis of NB:

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