Effects of vitamin E on lindane induced cytotoxicity in Chinese Hamster Ovary cell line (CROSBI ID 523973)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Kmetič, Ivana ; Šimić, Branimir ; Kniewald, Jasna
engleski
Effects of vitamin E on lindane induced cytotoxicity in Chinese Hamster Ovary cell line
Chinese Hamster Ovary (CHO K1) cell line was introduced in order to reduce the expensive and regulated tests in different animal species usually used for the safety assessment of pesticides. Lindane (gamma-hexachlorocyclohexane) is an insecticide with restricted permission of usage, declared as a highly toxic compound and their residues in the environment may present also a risk for the reproductive processes. The in vitro effects of the antioxidant alpha-tocopherol, vitamin E, on lindane induced cytotoxicity was studied by two different bioassays: Trypan Blue exclusion method and Kenacid Blue method. The cells were maintained at 37 ˚ C in an atmosphere of 95% air and 5% CO_2 in the medium Dulbecco’ s MEM supplemented with 5% newborn calf serum. The biomass was produced in T-bottles, cells were separated in the early logarithmic phase of growth and seeded on 6-multiwell plates. Initial concentration of CHO cells was 2x10^4 cells/mL/well. Target cells were preincubated with two different concentrations of vitamin E (25 micro g/mL and 50 micro g/mL) for 24 hours, and then treated up to 100 micro g/ml of lindane. Cytotoxicity of lindane was determined after 72 hours in the presence or absence of vitamin E. The applied concentration of lindane markedly inhibited the cell growth. When target cells were preincubated with higher dose of vitamin E (50 micro g/mL), the cell viability was significantly enhanced compared to the observed results in cells exposed only to lindane. These results suggest that vitamin E may exert a protective role in cell defence against lipophilic xenobiotics such as lindane.
lindane; CHO-K1 cell line; cytotxicity; Trypan Blue; Kenacid Blue; vitamin E protective role
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Podaci o prilogu
S218-x.
2006.
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objavljeno
Podaci o matičnoj publikaciji
Toxicology letters
Kehrer, J.P. ; Dekant, W. ; Smith, C. ; Kniewald, J. , guest editor
Amsterdam: Elsevier
0378-4274
Podaci o skupu
The EUROTOX 2006/6 CTDC Congress - the 43rd Congress of the European Societies of Toxicology and the 6th Congress of Toxicology in Developing Countries
poster
20.09.2006-24.09.2006
Cavtat, Hrvatska