Concentrations of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, smokers and COPD patients (CROSBI ID 525155)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Marević, Sanja ; Petrik, Jozsef ; Vrkić, Nada ; Popović-Grle, Sanja ; Čepelak, Ivana
engleski
Concentrations of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, smokers and COPD patients
COPD (chronic obstructive pulmonary disease) is a chronic progressive inflammatory disease characterized by limitations in lung airflow that is not fully reversible. Although the pathophysiology of COPD is not understood completely, it is well known that various cells in the lungs are releasing inflammatory mediators. Tobacco smoke induces the release of a pro-inflammatory cytokine TNF-alpha (tumor necrosis factor-alfa) that might play a key role in COPD. TNF-alpha activates certain transcription factors and switches on the transcription of genes for cytokines, chemokines, proteases and endothelins. CXCL8 (interleukin-8) is a chemokine that attracts neutrophils to the inflammatory site. ET-1 (endothelin-1) and big ET-1 contribute to pulmonary vasoconstriction and pulmonary hypertension in COPD patients. hsCRP (highly sensitivity C-reactive protein) is an acute phase protein elevated in inflammatory diseases. The aim of this study was to investigate the concentrations and correlation of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, healthy smokers and patients with COPD. The concentration of TNF-alpha and CXCL8 in serum and big ET-1 in plasma was measured with an ELISA test in healthy non-smokers (n=23, control group), healthy smokers (n= 30) and COPD patients (n=30). hsCRP was measured in serum nephelometrically. The concentration of hsCRP was significantly higher in patients with COPD than in the control group (p=0.0004) but the concentrations of TNF-alpha (p=0.0788), CXCL8 (p=0.4839) and big ET-1 (p=0.3641) were not statistically different. There was no significant difference in the measured analytes between smokers and control group. Spearman coefficient of correlation between the concentrations of TNF- alpha and CXCL8 was r= 0.638 (P<0.0001). hsCRP was higher in COPD patients, which is in agreement with the fact that COPD is an inflammatory disease. hsCRP proved to be a more sensitive diagnostic parameter than TNF-alpha, CXCL8 and big ET-1 in the systemic circulation in patients with COPD.
chronic obstructive pulmonary disease; TNF-alpha; big-ET-1; hsCRP
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Podaci o prilogu
S141-S142-x.
2006.
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objavljeno
Podaci o matičnoj publikaciji
Biochemia medica
Topić, Elizabeta ; Čvorišćec, Dubravka
Zagreb: Medicinska naklada
1330-0962
Podaci o skupu
5th Croatian Congress of Medical Biochemists with international participation
poster
18.10.2006-22.10.2006
Poreč, Hrvatska