engleski

Human Papillomavirus types in adenocarcinoma, adenocarcinoma in situ and adenosquamous

The epidemiology of adenocarcinoma (AC) of the cervix has changed substantially over the past decades. Recent reports indicate that the AC accounts for 20-25% (relative to squamous cell carcinoma (SCC)) in uterine cervical cancer comparing to only 5-15% in the past. It was noted that incidence data show steady increase in the rate of AC especially in younger women as well as for women 30-45 years of age. The role of human papilloma virus (HPV) infection in development of cervical carcinoma is well established but HPV type prevalence in cervical adenocarcinoma varies from study to study. It is generally believed that the HPV 18 is more frequently associated with cervical AC in contrast to HPV 16 in cervical SCC. We have conducted preliminary study in Rijeka region, Croatia to examine frequency of HPV types in AC, adenocarcinoma in situ (AIS) and adenosquamous carcinoma (ADSQ). Thus, DNA was isolated from archival material ; formalin fixed, paraffin embedded tissue histologically classified as AC (n=36), AIS (n=16), ADSQ (n=5) and HPV testing was performed by PCR using generic and genotype specific primers. Our preliminary data show increase of glandular cervical carcinomas during 1995-2005 period of time with predominant number of cases in women 29-49 years of age. HPV 18 predominates in AC, AIS whereas type HPV 16 is predominant in ADSQ. Following HPV types found were HPV-X, HPV-16 and multiple infections with different combinations of HPV18, HPV16, HPV31 and HPV33. Single infections (infection with only one HPV type) predominate in all three histological groups over multiple (infection with more than one HPV type) infections. Clearly little information is available about geographic variation of HPV types in AC therefore more research is needed to elicidate the role of different HPV types in development of AC.

cervical carcinoma; human papillomavirus; prevalence and genotypes

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