Ganglioside expression in tissues of mice deficient in beta2-microglobulin (CROSBI ID 470313)
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Podaci o odgovornosti
Markotić, Anita ; Marušić, Ana ; Jonjić, Stipan ; Mesarić, Marko ; Muething, Johannes
engleski
Ganglioside expression in tissues of mice deficient in beta2-microglobulin
Aim. To investigate ganglioside expression in lymphoid (spleen, thymus, and lymph nodes) and other tissues (lungs, brain, muscle, liver) of mice deficient in beta2-microglobulin (B2MG), component of the histocompatibility class I antigens (MHC I). Methods. Gangliosides were isolated from C57BL/6 mice homozygous (-/-) or heterozygous (-/+) for the B2MG gene knockout and separated by high pereformance thin-layer chromatography (HPTLC). Individual gangliosides were visualized using resorcinol stain and HPTLC immunostainig overlay technique with polyclonal antibodies specific for GM3(Neu5Ac), GM3(Neu5Gc), GalNAcGM1b, and nLc4. GM1b-type gangliosides were detected after V. cholerae neuraminidase treatment with a specific anti-Gg4 antibody. Neuraminidase pretreatment was also used to detect gangliosides IV3nLc4 and VI3nLc6. GM1a and Gal-GalNac-GM1b gangliosides were detected with choleragenoid. Individual HPTLC bands were scanned with a CD60 scanner to quantify the differences between -/- and -/+ mice. Results. GM3(NeuAc) was four times and GM3(Neu5Gc) three times more abundant in the lungs of -/+ compared to -/- mice. Two bands of GM1b, GalNac-GM1b, and Gal- GalNac-GM1b, respectively, were expresses in the lungs of -/+ mice, but not in the lungs of -/- mice. Gangliosides of the neolacto-series were several times more abundant in the muscle, spleen, thymus, and lymph nodes of -/+ mice than of -/- mice. Conclusion. Our study provides in vivo evidence that B2MG is involved in the acyusition of distinct ganglioside profiles of different mouse organs.
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Podaci o prilogu
L 14, 45-x.
1998.
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objavljeno
Podaci o matičnoj publikaciji
Annual meeting of Croatian biochemists with international participation
Zagreb:
Podaci o skupu
Annual Meeting of Croatian Biochemists with International Participation
poster
17.09.1998-20.09.1998
Bizovac, Hrvatska