engleski

Nascent ribosome biognesis is required during cell growth

To gain insight into importance of this process during mouse oogenesis and embriogenesis we deleted one allele of the ribosomal protein S6 gene in growing oocytes and generated S6-heterozygous embryos. Oogenesis and embryonic development untill E5.5 were normal.However, inhibition of entry into Mphase of the cell cycle and apoptosis became evident post E5.5, leading to perigastrulation lethality.Genetic inactivation of p53 bypassed this checkpoint and prolonged development until E12.5, when they die with defective translation of D-type cyclins and erythropoesis.Thus, a p53 dependent checkpoint is activated during gastrulation in response to ribosome insufficiency in order to prevent improper execution of the developmental program.

ribosome biogenesis; S6-haploinsufficiency; p53-dependent checkpoint

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