engleski

Rapid Extraction of Human DNA containing humic acid

The identification process of dead bodies or human remains is nowdays conducted in numerous fields of forensic science, archeology and other juditial cases. A particular problem is the isolation and DNA typing of human remains found in mass graves, due to the degradation process, as well as post mortal DNA contamination with bacteria, fungi, humic acids, metals etc. In this study we investigated the influence of humic acid (HA) on the DNA extraction and typing. We reported that the humic acid if present in the amplification reaction mix inhibited the DNA amplificatio, but the addition of 50 mg PVPP to the reaction mixture appeared to be optimum in overcoming that inhibition.

human DNA; extraction; humic acid; DNA inhibition

nije evidentirano