engleski

Oxidative Stress in Mice: Effects of Dietary Corn Oil and Iron

The aim of this study was to analyze the effect of high dietary iron (Fe) on liver antioxidant status in mice fed corn oil-enriched diet. Male Balb/c mice were fed for 3 wk standard diet enriched with 5% by weight of corn oil with adequate Fe (FCO), or supplemented with 1% carbonyl Fe (FCOFe diet). Control group was fed standard diet. The high-Fe diet induced twofold increase of hepatic Fe level. However, an increase of thymic Fe level has been induced solely by dietary fat. The hepatic copper (Cu) level slightly decreased in FCO diet. In the spleen, the high-Fe diet induced increase of Fe level was negatively correlated with Cu level. The antioxidant status was influenced by both dietary fat and Fe. Mice fed corn oil-enriched diets had higher concentration of thiobarbituric acid-reactive substances (TBARS), with a greater increase in FCOFe diet. Fatty acid analysis showed decreased n-3 and n-6/n-3 ratio, particularly in FCOFe diet. Hepatic Cu/Zn superoxid dismutase (CuZn-SOD) activity was decreased in FCO diet, while Fe supplementation caused its further decrease. These results suggest that feeding with corn oil-enriched diet increases oxidative damage by decreasing antioxidant enzyme defence. The high-Fe diet has affected the antioxidant defence system, further increasing the tissue’ s susceptibility to lipid peroxidation. Additionally, both corn oil- and Fe-enriched diets have increased the Cu requirement in mice.

corn oil; iron; copper; zinc; superoxid dismutase; glutathione peroxidase; thiobarbituric acid reactive substances

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