engleski

Purification of plant viral and satellite double stranded RNAs on DEAE monoliths

Replicative double-stranded RNA (dsRNA) is useful in preliminary identification of Cucumber mosaic virus and its satellite RNA. This plant pathogen complex yields sufficient quantity of the replicative RNA form that can be isolated by CF-11 cellulose chromatography. In this work, much faster and more efficient procedure using monoliths was developed in which dsRNA was separated from other species in total nucleic acids extract originating from the infected plant tissue. The developed chromatographic method revealed the pathogens' presence in only 15 minutes, avoiding nucleic acid precipitation and electrophoretic analysis.

convective interaction media; cucumber mosaic virus (CMV); CMV satRNA; preliminary diagnosis

2nd Summer School on Monolith Technology for Biochromatography, Bioconversion and Solid-Phase Synthesis ; Portorož, Slovenia, 28-31 May 2006.