Comparison of the L-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells (CROSBI ID 132247)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vrsalović Presečki, Ana ; Zelić, Bruno ; Vasić-Rački, Đurđa
engleski
Comparison of the L-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells
Production of L-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized bakers yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch experiments. It was found that this reaction is inhibited by the product. Equilibrium conversion of about 80 % was achieved in tha batch mode by both biocatalysts. No by-product was detected during the L-malic production by permeabilized yeast cell. It was calculated by using the proposed model and experimentally verifies that 10 mg isolated fumarase gives the identical productivity in this biotransformation as 94.4 g baker yeast
L-malic acid ; fumarase ; baker yeast ; permeabilization ; modelling
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Podaci o izdanju
41 (5)
2007.
605-612
objavljeno
0141-0229
1879-0909
10.1016/j.enzmictec.2007.05.007
Povezanost rada
Biotehnologija, Kemijsko inženjerstvo