engleski

Renal expression of organic anion transporter OAT2 in rats and mice is regulated by sex hormones

The renal reabsorption and/or excretion of various organic anions is mediated by specific transporters (OATs). OAT2 (Slc22a7) has been identified in rat kidney, where its mRNA expression exhibits gender differences (females (F)>males (M)). The exact localization of OAT2 protein in the mammalian kidney has not been reported. Here we studied the expression of OAT2 mRNA by RT-PCR and its protein by Western blotting (WB) and immunocytochemistry (IC) in kidneys of adult intact and gonadectomized M and F, sex hormone-treated castrated M, and prepubertal M and F rats, and the protein in adult M and F mice. In adult rats, the expression of OAT2 mRNA was predominant in the outer stripe (OS) tissue, exhibiting: a) gender dependency (F>M), b) upregulation by castration and downregulation by ovariectomy, and c) strong downregulation by testosterone-, and weak upregulation by estradiol- and progesterone- treatment. A polyclonal antibody against the rat OAT2 on WB of isolated renal membranes labeled a ~66 kDa protein band, that was stronger in F. By IC, the antibody exclusively stained brush-border (BB) of the proximal tubule S3 segment (S3) in the OS and medullary rays (F>M). In variously treated rats, the pattern of 66 kDa band density in the OS membranes and the staining intensity of BB in S3 matched the mRNA expression. The expression of OAT2 protein in prepubertal rats was low and gender-independent. In mice, the expression pattern largely resembled that in rats. Therefore, OAT2 in rat (and mouse) kidney is localized to the BB of S3, exhibiting gender differences (F>M) that appear in puberty and are caused by strong androgen inhibition and weak estrogen and progesterone stimulation.

androgens ; estrogens ; gender differences ; kidney ; membrane transporters ; progesterone ; organic anion ; transporter-2

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