engleski

Determination of mite allergens in house dust using the enzyme immunoassay

The aim of this study was to determine the level of Dermatophagoides pteronyssinus (Der p 1) and Dermatophagoides farinae (Der f 1) major mite allergens in 30 urban homes in Zagreb, Croatia, using the enzyme immunoassay with two monoclonal antibodies which has been established as the reference method for indoor allergen analysis. Dust samples were taken by vacuuming a carpeted area and collected on cellulose filters. The ranges of Der p 1 and Der f 1 were 0.1-12.5 &micro ; g/g (median 0.32 &micro ; g/g) and 0.1-31.2 &micro ; g/g (median 0.35 &micro ; g/g), respectively. There was a large range in Der p 1 and Der f 1 concentrations (>102 &#8211; fold). Der p 1 and Der f 1 concentrations (> 2 &micro ; g/g) associate with increased risk of sensitization to mite allergens were found in approximately 16 % homes, each. The sum of allergen (Der p 1 + Der f 1) exceeded the lower threshold in 27 % homes. Analytical evaluation of ELISA assay showed satisfactory results for precision (intra-assay CV < 6.9%, inter-assay CV<13.3%), accuracy (91-93%) and sensitivity (2 ng/mL). The ELISA assay for the measurement of dust mites allergen demonstrated very good analytical characteristics for routine laboratory use, and will provide the essential basis for our future studies of various indoor allergens.

house dust mites; indor allergens; Der p 1; Der f 1; ELISA

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