engleski

Optimization of the TLC Separation of Seven Amino Acids

A ternary mobile phase for TLC separation and identification of seven amino acids on microcrystalline cellulose, with nynhidrin as detection reagent, has been optimized by use of the experimental design software packages Design-Expert 6 and Statistica. When the results from each product were compared the optimum mobile phase was found to be butanol-glacial acetic acid-water 60.14:18.77:21.09. The performance of this mobile phase was confirmed experimentally.

thin layer chromatography; optimization; amino acids

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