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Pregled bibliografske jedinice broj: 303740

Časopis

Autori: Wolstencroft, Elizabeth; Šimić, Goran; thi Man, Nguyen; Holt, Ian; Lam, Le Than; Buckland, Paul; Morris, E. Glenn
Naslov: Endosomal location of dopamine receptors in neuronal cell cytoplasm
Izvornik: Journal of Molecular Histology (1567-2379) 38 (2007), 4; 333-340
Vrsta rada: članak
Ključne riječi: dopamine; dopamine receptors; G protein-coupled receptor; endosome; NT-2 cells; Ntera-2; monoclonal antibody; brain; hippocampus; cerebellum; cerebral cortex; mannose-6-phosphate receptor
Sažetak:
Five subtypes of dopamine receptor exist in two subfamilies: two D(1)-like (D(1) and D(5)) and three D(2)-like (D(2), D(3) and D(4)). We produced novel monoclonal antibodies against all three D(2)-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most of the immunostaining for all three receptors colocalized with mannose-6-phosphate receptor, a marker for late endosomes formed by internalization of the plasma membrane. This result was obtained with antibodies against three different epitopes on the D(3) receptor, to rule out the possibility of cross-reaction with another protein, and controls without primary antibody or in the presence of competitor antigen were completely negative. In rat cerebral cortex and hippocampus, some of the dopamine receptor staining was found in similar structures in neuronal cell cytoplasm. Only some of the neurons were positive for dopamine receptors and the pattern was consistent with previously-reported patterns of innervation by dopamine-producing neurons. Endosomal dopamine receptors may provide a useful method for identifying cell bodies of dopamine-responsive neurons to complement methods that detect only active receptors in the neuronal cell membrane.
Projekt / tema: 108-1081870-1942
Izvorni jezik: ENG
Current Contents: DA
Citation Index: DA
Ostale indexne publikacije: Biological Abstracts;Chemical Abstracts;Excerpta Medica;Index Medicus;BIOSIS;CABS;IBIDS;ISI Alerting Services;Science Citation Index Expanded;SCOPUS
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti,Psihologija
Puni text rada: 303740.fulltext.pdf (tekst priložen 20. Kol. 2007. u 12:41 sati)
Tiskani medij: da
URL Internet adrese: http://www.springerlink.com/content/353t21168pq07477/fulltext.pdf
Broj citata:
Altmetric:
DOI: 10.1007/s10735-007-9106-5
Google Scholar: Endosomal location of dopamine receptors in neuronal cell cytoplasm
Upisao u CROSBI: Goran Šimić (gsimic@mef.hr), 20. Kol. 2007. u 12:20 sati



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