engleski

Effect of organic selenium and 48 h post-growing fast on the prooxidant/antioxidant system in heart muscle of broiler chickens

The objective of the experiment was to determine the effects of organic selenium feed supplements and the 48 h post-growing fast on the antioxidant enzymes activity and lipid peroxidation in the chicken heart muscle. The experiment was carried out on the Ross 308 broiler chickens. During the growing period one group was fed with standard diet (control), and in another group of chicken 0.3 ppm of organic selenium was added in the standard diet (experimental). After the growing and 48-h fasting period, 10 chickens from each group were randomly selected and slaughtered. The activity of glutathione peroxidase (GSH-Px), catalase (CAT), cooper zinc superoxide dismutase (Cu, Zn-SOD), manganese superoxide dismutase (Mn-SOD), and reduced glutathione (GSH) and lipid peroxide (TBARS) concentration was determined in the heart muscle from both group of chickens. The activity of CAT and Mn-SOD was significantly higher after the fasting period in both group of chickens (P<0.0001 and P<0.01 respectively for control and experimental treatments). After the fasting period, GSH-Px activity was significantly higher in chickens with organic selenium supplementation than in the chicken fed with standard diet (P<0.02). There were no statistically significant differences observed in Cu, Zn-SOD activity or in GSH and TBARS concentration between the control and experimental group. The results show that after the fasting period, chickens with organic selenium supplementation, maintained the antioxidant system in the heart muscle more efficiently, with more intense GSH-Px antioxidant defence. These data suggest that organic selenium supplementation may benefit feed restricted broiler breeders.

organic selenium; fasting; heart muscle; chicken

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