engleski

NF-κ B pathway in F. tularensis infections

Francisella tularensis has been shown to induce apoptosis within murine and human macrophages. Several pathogens have been shown to promote or interfere with apoptosis through the inhibition or activation of nuclear transcription factor B (NF-κ B). The mechanisms and significance of F. tularensis-associated apoptosis through NF-κ B are not well understood. To examine nuclear translocation of the p65 subunit and apoptosis in F. tularensis-infected cells, monolayers of human monocytes derived macrophages (hMDMs) were infected with wt F. tularensis subsp. novicida and proceed for confocal microscopy analyses. To examine the effect of caffeic acid phenethyl ester (CAPE) on F. tularensis-induced nuclear translocation of the p65 subunit, hMDMs were treated with CAPE for 30 min prior to infection. At 30 min, 1, 6, 24 and 48 h after infection, 83%, 96%, 44%, 43% and 35% of F. tularensis-infected macrophages, respectively, showed nuclear translocation of the p65 subunit. Our data also showed that nuclear translocation of the p65 subunit was detected in only ~15% of F. tularensis-infected macrophages in the presence of CAPE. TUNEL labeling for apoptotic nuclei revealed that in CAPE-treated cells 25% of F. tularensis subsp.novicida-infected cells were apoptotic. We conclude that the mechanism of sustained nuclear translocation of the p65 subunit in F. tularensis infection is cruical at the later stage of infection. Our data show that the activation of NF-κ B plays a role in protecting the infected cells from external apoptotic stimuli to maintain viability of the infected cells.

apoptosis; NF-kappa B; Francisella

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