engleski

Age-related Changes in the Serum Dipeptidyl Peptidase IV (DPP IV/CD26) Activity in Patients with Inflammatory Bowel Diseases

Inflammatory bowel diseases (IBDs) represent a group of idiopathic diseases which affect the intestine and include two clinical subtypes: Crohn's disease (CD) and ulcerative colitis (UC). The etiology and pathogenesis of IBD still not understood well. The dipeptidyl peptidase IV (DPP IV/CD26) is a membrane-bound multifunctional glycoprotein, acting as a proteolytic molecule, receptor, binding and costimulatory molecule. The proteolytic cleavage of the membrane bound DPP IV results in a soluble form that migrates in the plasma. It has previously been shown that the DPP IV plays a significant role in the pathophysiology of IBD. The aim of this study was to determine the influence of patient's age on the serum dipeptidyl peptidase IV (DPP IV/CD26) activity in patients affected with IBD. The research was performed on 93 patients, divided in 2 groups: 31 young patients (mean age 13, 8 +/- 1, 7 years, 24 with CD and 7 with UC) and 62 adult patients (age 42, 7 +/- 14, 4 years, 38 with CD and 24 with UC). The control group included 111 healthy blood donors: 46 children (age 13, 8 +/- 2, 8 years) and 65 adults (age 41, 6 +/- 12, 1 years). The serum DPP IV activity has been measured spectrofotometrically at 405 nm by detection of free 4-nitroaniline in an assay mixture using Gly-Pro p-nitroanilide as substrate. The serum DPP IV activities in both young and adult patients with IBD were found to be statistically sigificantly decreased when compared to their healthy controls. The values correlated inversely with the disease severity for both CD and UC. When comparing serum DPP IV activities between young and adult patients with IBD, but even between young and adult healthy controls, it is clear that the serum DPP IV activity decreases statistically significantly with age. The results of this study show that the serum DPP IV could be useful as an available, non-invasive marker in the diagnosis of the disease activity. This research clearly shows that age-related standard values should be undoubtedly established in clinical laboratory practice because of its age-dependent decrease in serum activity.

Dipeptidyl Peptidase IV (DPP IV/CD26); Serum Activity; Inflammatory Bowel Diseases; Age

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