engleski

Laccase catalysed oxidation of L-DOPA in a microreactor

Laccases (EC 1.10.3.2 p-diphenol: dioxygen oxidoreductases) are multi-copper proteins that use molecular oxygen to oxidize various aromatic and non-aromatic compounds by radical-catalyzed reaction[1]. Their applications are numerous: delignification or decolouration of paper pulp, detoxification of environmental pollutants, textile dye-bleaching or diagnostic assays[2]. The fields of application of enzymatic microreactors grow every day. Their current use is primarily in biochemical analysis as components of integrated systems (lab-on-a-chip) or in micro total analysis systems - &micro ; ; TAS. Secondly, microreactors are used for screening of substrates, enzymes and to examine their kinetic characteristics. However, their huge potential is not yet exploited in biotechnology as well as in combinatorial chemistry and enzyme-targeted drug search[3]. In this work, the use of microreactor for oxidation of phenolic compounds was investigated. L-DOPA was selected as a model substrate for laccase-catalysed oxidation, which was carried out in a microreactor (220 &micro ; ; m X 50 &micro ; ; m X 0.332 cm) at four different residence times. Process model, comprised of reaction diffusion dynamics combined with fluid flow was developed. The simulation of the process for different substrate and enzyme concentrations, as well as for different flow rates was performed. A very good correlation of experimental data with the proposed model was achieved. Literature: [1] Claus H.: Laccases: structure, reactions, distribution ; Micron 35 (2004), 93-96 [2] Pilz R., Hammer E., Schauer F., Kragl U.: Laccase – catalysed of coupling products of phenolic substrates in different reactors: Appl Microbiol 60 (2003), 708-712 [3] Urban, P. L., Goodall D. M., Bruce N. C.: Enzymatic microreactors in chemical analysis and kinetic studies, Biotechnol Adv 24 (2006), 42-57

L-DOPA; laccase; microreactor

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