engleski

In vitro proliferation of lymphocytes derived from pigs vaccinated against classical swine fever

The aim of this study was to evaluate the in vitro influence of mitogens on the lymphoproliferative response after vaccination against classical swine fever (CSF). We also compared the effect of the ORF virus strain D1701, whose immunostimulatory effects in vivo are known, as well as the CSF virus C strain on lymphocyte cell cultures of vaccinated and control pigs. Peripheral blood leucocytes (PBL) were used in a lymphocyte proliferation assay by Concanavalin A, phytohemagglutinin, pokeweed mitogen, the attenuated ORF virus strain D1701 and the CSF virus China (C) strain. PBL were obtained from pigs vaccinated with different CSF vaccines (a subunit gp E2 vaccine, a combination of a subunit vaccine and an ORF virus strain D1701 and attenuated C strain vaccine) and from non-vaccinated pigs. Blood samples for the mitogenic response assay were collected before (day 0), and on the 4th, 10th, 21st and 28th post-vaccination day. PBL of pigs that received the C strain and ORF virus showed in vitro a weaker proliferative response to the mitogens than PBL of pigs vaccinated with the subunit CSF vaccine. The lymphocyte proliferation was observed only in pigs vaccinated with the C strain vaccine after exposure to the same strain in vitro. However, the ORF virus, in the applied concentration did not induce a significant reaction of lymphocytes that originated from either vaccinated or control animals. The results of our study suggest that the applied mitogens may induce in vitro reactivity which could play a significant immunological role after CSF vaccination. In contrary, the attenuated virus strains used as stimulators, induced no significant lymphoproliferation.

classical swine fever ; C strain ; lymphoproliferation ; mitogens ; ORF virus

Rad je kao poster prezentiran na skupu "7th International Congress of Veterinary Virology", održanom od 24-27.09.2006., Lisabon, Portugal, i uz međunarodnu recenziju objavljen kao sažetak u "Abstracts of the 7th International Congress of Veterinary Virology" ; Alexandre Letiao, Carlos Martinis (ur.) ; Lisabon, 2006. ; str. 256.