Size determination of liposome with incorporated ovalbumin using dynamic light scattering and fluorescence microscopy (CROSBI ID 529375)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Frkanec, Ruža ; Brgles, Marija ; Jurašin, Darija ; Dutour Sikirić, Maja ; Filipović-Vinceković, Nada ; Tomašić, Jelka
engleski
Size determination of liposome with incorporated ovalbumin using dynamic light scattering and fluorescence microscopy
In our previous studies we have demonstrated the interactions of ovalbumin (OVA) with lipids in liposomal membranes [1] and the effects of OVA encapsulated in liposomes on the immune response in mice [2]. The aim of this work was to investigate the size and zeta potential of large multilamellar liposomes with incorporated ovalbumin using dynamic light scattering and fluorescence microscopy. Multilamellar cationic liposomes consisting of phosphatidylcholine (PC): dioleoylphosphatidylethanolamine (DOPE) : 1, 2-Dioleoyl-3-trimethylammoniumpropane (DOTAP) and negatively charged liposomes of phosphatidylcholine: cholesterol (Chol): dicetylphosphate (DcPh) were prepared by the thin film hydration method. Different OVA quantities and different buffers for encapsulation OVA in liposomes were used. The rodamine B and fluorescein-isothiocianate were used for fluorescence measurements. The results revealed that the size of cationic liposomes in PBS increases linearly with increasing amount of OVA. The zeta-potential of examined liposomes preparations was slightly different.
liposomes; dynamic light scattering; ovalbumin; fluorescence microscopy
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Podaci o prilogu
41-41.
2007.
objavljeno
Podaci o matičnoj publikaciji
COST D27 action prebiotic chemistry and early evolution : final evoluatin workshop : book of abstracts
Nothig Laslo, Vesna
Zagreb: Institut Ruđer Bošković
978-953-6690-66-4
Podaci o skupu
Final Evaluation Workshop of COST D27 Action "Prebiotic Chemistry and Early Evolution"
poster
11.05.2007-13.05.2007
Dubrovnik, Hrvatska