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Designing a small-scale field experiment for treatment of PCB-contaminated soil (CROSBI ID 529519)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Petrić, Ines ; Hršak, Dubravka ; Fingler, Sanja Designing a small-scale field experiment for treatment of PCB-contaminated soil // 4th Symposium on Biosorption and Bioremediation : abstracts / Mackova, Macek ; Demnerova, Pazlar (ur.). Prag, 2007. str. 61-61

Podaci o odgovornosti

Petrić, Ines ; Hršak, Dubravka ; Fingler, Sanja

engleski

Designing a small-scale field experiment for treatment of PCB-contaminated soil

The overall objective of our study was to propose a bioremediation method for enhancing PCB degradation in contaminated soil of a transformer station in Croatia, damaged during warfare operations in 1991. After cycles of microcosm enrichments and detailed laboratory biodegradation experiments a mixed bacterial culture originating from PCB-contaminated soil in the vicinity of the transformer station and one of its members, both showing substantial PCB degradation activity, were selected as promising seed cultures for small-scale-field biodegradation experiment. For designing the experiment two strategies have been employed: 1) bioaugmentation, i.e. addition of the selected cultures along with soya lecithin (surfactant to enhance PCB bioavailability), xylose (supplemental carbon source) and carvone (inducer of PCB catabolism), and 2) biostimulation of indigenous soil microflora (addition of soya lecithin, xylose and carvone). Inoculation and amendment was repeated to maintain augmented culture density as well as soil moisture (30-40 %). Soil samples were analysed every 3 months for the changes in PCB mass fractions by GC/MS analyses, and the obtained results suggested that fourteen dominant peaks detected in contaminated soils that corresponded to tri- and tetrachlorobiphenyls were approximately 40 % reduced after 6-month treatment. GC/MS analyses revealed as well no marked differences between the bioaugmentation and the biostimulation treatment. During the experiment several biphenyl-degrading strains belonging to the genera Rhodococcus and Microbacterium have been isolated. Our results confirmed that PCB degradation in soil is a slow process ; however, gradual reduction of dominant PCB congeners is considered a good indication of progressive soil decontamination.

PCB; biodegradation

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Podaci o prilogu

61-61.

2007.

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objavljeno

978-80-7080-025-6

Podaci o matičnoj publikaciji

4th Symposium on Biosorption and Bioremediation : abstracts

Mackova, Macek ; Demnerova, Pazlar

Prag:

Podaci o skupu

Symposium on Biosorption and Bioremediation (4 ; 2007)

poster

26.08.2007-30.08.2007

Prag, Češka Republika

Povezanost rada

Biologija